Sequential Embryo Transfer

What is Sequential Embryo Transfer?

Sequential Embryo Transfer (SET) is a specialized IVF technique in which two sets of embryos are transferred into the uterus at different stages of the menstrual cycle. SET has gained widespread popularity globally and is now commonly practiced in India as well.

In this procedure, embryos that have been partially developed in the lab are transferred into the uterus—typically on days 2–3 and again on days 5–6 of the menstrual cycle. This method aims to improve implantation by allowing the most optimally developed embryo to attach to the uterine lining, thereby enhancing the chances of a successful pregnancy.

The effectiveness of sequential embryo transfer also depends on several lifestyle factors. Patients are encouraged to follow a nutritious diet, engage in regular physical activity, and take recommended natural vitamin supplements to support the process and improve results.

What Is The Procedure of Sequential Embryo Transfer?

Initially, two batches of lab-cultured embryos are transferred into the mother’s uterus during the same menstrual cycle-first on days 2-3 and then on days 5-6.
The first batch, consisting of fast-growing, good-quality embryos, is transferred after 2-3 days of development.
A few days later, the second batch of healthy, well-developed embryos is transferred, typically on days 5-6. By this stage, these embryos are more mature and often reach the blastocyst stage of development.
Due to their advanced maturity, the second set of embryos tends to attach more effectively to the uterine lining. They begin releasing cytokines, which help enhance the uterine environment and promote successful implantation.